| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reznick, Cole T (17)
|
PCTRIV |
49.50 |
49.59 |
0.09 |
| 2 |
Alarcon, Greyson B (17)
|
STNTRO |
50.21 |
50.57 |
0.36 |
| 3 |
Ries, Joshua O (17)
|
UTHAST |
50.79 |
50.92 |
0.13 |
| 4 |
Wu, Eric Y (16)
|
ORCRST |
50.83 |
51.03 |
0.20 |
| 5 |
Koster, Jeremiah D (16)
|
CCTNT |
51.77 |
51.48 |
-0.29 |
| 6 |
Willstrop, Samuel J (17)
|
STAAAA |
53.21 |
51.50 |
-1.71 |
| 7 |
Ascencio, Ryan C (17)
|
CAMVN |
51.49 |
51.59 |
0.10 |
| 8 |
Liu, Trevor K (17)
|
NTMTRO |
51.80 |
51.69 |
-0.11 |
| 9 |
Petty, Alexander J (16)
|
MWLSS |
52.22 |
51.74 |
-0.48 |
| 10 |
Vaughn, James O (16)
|
NTMTRO |
50.74 |
51.77 |
1.03 |
| 11 |
Eckerman, Brendan E (17)
|
COACES |
53.90 |
51.78 |
-2.12 |
| 12 |
Hamilton, Casey W (17)
|
AKGSC |
51.74 |
51.97 |
0.23 |
| 13 |
Collins, Leo D (16)
|
PCNBA |
52.61 |
52.17 |
-0.44 |
| 14 |
Stone, Alexander H (14)
|
IAL4A |
55.32 |
55.59 |
0.27 |
|
Gray, Andrew A (16)
|
MNEDI |
50.00 |
NS |
- |
|
Baginski, Nicholas S (17)
|
ILCATS |
|
NS |
- |
|
Sego, Ryan (17)
|
ILACAD |
50.79 |
NS |
- |